Molecular cloning and chromatin structure analysis of the murine α 1(I) collagen gene domain
Identifieur interne : 000C99 ( Main/Exploration ); précédent : 000C98; suivant : 000D00Molecular cloning and chromatin structure analysis of the murine α 1(I) collagen gene domain
Auteurs : Payman Salimi-Tari [États-Unis] ; Michèlle Cheung [États-Unis] ; Caroline A. Safar [États-Unis] ; John T. Tracy [États-Unis] ; Isabel Tran [États-Unis] ; Klaus Harbers [Allemagne] ; Michael Breindl [États-Unis]Source :
- Gene [ 0378-1119 ] ; 1997.
Abstract
We have isolated molecular clones of genomic mouse DNA spanning 55kb, including the entire coding region of the murine α1(I) collagen (Col1a1) gene and 24kb of 5′ and 13kb of 3′-flanking sequences, and have performed a detailed chromatin structure analysis of these sequences. Several new DNase-I-hypersensitive sites were identified. The distal 5′-flanking region contains two clusters of DNase-I-hypersensitive sites located between 7 and 8kb and between 15 and 20kb upstream of the start site of transcription, respectively. Several of these sites were shown to be present in collagen-producing, but not in non-producing cells, indicating that they are associated with transcription of the gene and may function in its regulation. One strong constitutive DNase-I-hypersensitive site at −18.5kb was also cleaved by endogenous nucleases. The 3′-flanking region of the gene contains a DNase-I-hypersensitive site located 6kb downstream of the end of the gene, as well as sequences that can induce a non-B DNA structure. Because these latter sequences coincide with DNase-I-hypersensitive sites in the homologous human gene, our results suggest that some regulatory elements may play a role in gene regulation, not by specific protein–DNA interactions but by virtue of their ability to induce a non-B DNA structure and/or an alternate chromatin conformation. A comparison of the murine and human Col1a1 domains shows a similar, although not identical, distribution of DNase-I-hypersensitive sites, indicating a conserved arrangement of regulatory elements. Our results strongly suggest that these new sites constitute regulatory elements which are involved in the transcriptional regulation and/or chromatin loop organization of the Col1a1 gene, and they are now amenable for functional analyses.
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DOI: 10.1016/S0378-1119(97)00293-X
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Tracy</name></author><author><name sortKey="Tran, Isabel" sort="Tran, Isabel" uniqKey="Tran I" first="Isabel" last="Tran">Isabel Tran</name></author><author><name sortKey="Harbers, Klaus" sort="Harbers, Klaus" uniqKey="Harbers K" first="Klaus" last="Harbers">Klaus Harbers</name></author><author><name sortKey="Breindl, Michael" sort="Breindl, Michael" uniqKey="Breindl M" first="Michael" last="Breindl">Michael Breindl</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:317543F2A1BF2B520FEE87D74CDBB3F3CDA1D6E8</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1016/S0378-1119(97)00293-X</idno><idno type="url">https://api.istex.fr/document/317543F2A1BF2B520FEE87D74CDBB3F3CDA1D6E8/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000816</idno><idno type="wicri:Area/Main/Curation">000811</idno><idno type="wicri:Area/Main/Exploration">000C99</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Exploration">000C99</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Molecular cloning and chromatin structure analysis of the murine α 1(I) collagen gene domain</title><author><name sortKey="Salimi Tari, Payman" sort="Salimi Tari, Payman" uniqKey="Salimi Tari P" first="Payman" last="Salimi-Tari">Payman Salimi-Tari</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology and Molecular Biology Institute, San Diego State University, San Diego, CA 92182</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Cheung, Michelle" sort="Cheung, Michelle" uniqKey="Cheung M" first="Michèlle" last="Cheung">Michèlle Cheung</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology and Molecular Biology Institute, San Diego State University, San Diego, CA 92182</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Safar, Caroline A" sort="Safar, Caroline A" uniqKey="Safar C" first="Caroline A." last="Safar">Caroline A. 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Several new DNase-I-hypersensitive sites were identified. The distal 5′-flanking region contains two clusters of DNase-I-hypersensitive sites located between 7 and 8kb and between 15 and 20kb upstream of the start site of transcription, respectively. Several of these sites were shown to be present in collagen-producing, but not in non-producing cells, indicating that they are associated with transcription of the gene and may function in its regulation. One strong constitutive DNase-I-hypersensitive site at −18.5kb was also cleaved by endogenous nucleases. The 3′-flanking region of the gene contains a DNase-I-hypersensitive site located 6kb downstream of the end of the gene, as well as sequences that can induce a non-B DNA structure. Because these latter sequences coincide with DNase-I-hypersensitive sites in the homologous human gene, our results suggest that some regulatory elements may play a role in gene regulation, not by specific protein–DNA interactions but by virtue of their ability to induce a non-B DNA structure and/or an alternate chromatin conformation. A comparison of the murine and human Col1a1 domains shows a similar, although not identical, distribution of DNase-I-hypersensitive sites, indicating a conserved arrangement of regulatory elements. Our results strongly suggest that these new sites constitute regulatory elements which are involved in the transcriptional regulation and/or chromatin loop organization of the Col1a1 gene, and they are now amenable for functional analyses.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>Californie</li><li>Hambourg</li></region><settlement><li>Hambourg</li></settlement></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Salimi Tari, Payman" sort="Salimi Tari, Payman" uniqKey="Salimi Tari P" first="Payman" last="Salimi-Tari">Payman Salimi-Tari</name></region><name sortKey="Breindl, Michael" sort="Breindl, Michael" uniqKey="Breindl M" first="Michael" last="Breindl">Michael Breindl</name><name sortKey="Cheung, Michelle" sort="Cheung, Michelle" uniqKey="Cheung M" first="Michèlle" last="Cheung">Michèlle Cheung</name><name sortKey="Safar, Caroline A" sort="Safar, Caroline A" uniqKey="Safar C" first="Caroline A." last="Safar">Caroline A. Safar</name><name sortKey="Tracy, John T" sort="Tracy, John T" uniqKey="Tracy J" first="John T." last="Tracy">John T. Tracy</name><name sortKey="Tran, Isabel" sort="Tran, Isabel" uniqKey="Tran I" first="Isabel" last="Tran">Isabel Tran</name></country><country name="Allemagne"><region name="Hambourg"><name sortKey="Harbers, Klaus" sort="Harbers, Klaus" uniqKey="Harbers K" first="Klaus" last="Harbers">Klaus Harbers</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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